1RSG
Crystal structure of the polyamine oxidase Fms1 from yeast
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979106 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.385, 103.126, 77.590 |
| Unit cell angles | 90.00, 94.98, 90.00 |
Refinement procedure
| Resolution | 35.950 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.206 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SAPI |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.302 |
| Number of reflections | 96862 | |
| Completeness [%] | 96.7 | 73 |
| Redundancy | 6.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 3350, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 3350, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
