1RRP
STRUCTURE OF THE RAN-GPPNHP-RANBD1 COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04 |
| Detector | SIEMENS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.339, 139.463, 91.654 |
| Unit cell angles | 90.00, 135.78, 90.00 |
Refinement procedure
| Resolution | 12.500 - 2.960 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RAN-GDP |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.300 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.091 | 0.191 |
| Total number of observations | 90705 * | |
| Number of reflections | 20286 | |
| <I/σ(I)> | 13.1 | 3.1 |
| Completeness [%] | 86.0 | 66 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.25 | pH 6.25 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 18 (%) | |
| 2 | 1 | 1 | ammonium sulfate | 250 (mM) | |
| 3 | 1 | 1 | MES | 100 (mM) |
