1RQ0

Crystal structure of peptide releasing factor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	90
Detector technology	CCD
Collection date	2001-06-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	51.900, 137.230, 80.930
Unit cell angles	90.00, 106.00, 90.00

Refinement procedure

Resolution	19.890 - 2.650
R-factor	0.216
Rwork	0.216
R-free	0.29400
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.700	2.700
High resolution limit [Å]	2.650	2.650
Number of reflections	30338
<I/σ(I)>	12.9
Completeness [%]		84.2
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 3350, KCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K