1RQ0
Crystal structure of peptide releasing factor 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2001-06-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.900, 137.230, 80.930 |
Unit cell angles | 90.00, 106.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 2.650 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.29400 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.700 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 30338 | |
<I/σ(I)> | 12.9 | |
Completeness [%] | 84.2 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 3350, KCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |