1RPW
Crystal Structure Of The Multidrug Binding Protein Qacr Bound To The Diamidine Hexamidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 171.800, 171.800, 94.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.980 - 2.900 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28000 |
| Structure solution method | Difference Fourier methods |
| Starting model (for MR) | 1jt6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.980 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.053 | 0.380 |
| Number of reflections | 31376 | |
| <I/σ(I)> | 6624 | 3.3 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.62 | 295 | Ammonium Sulfate, Sodium Acetate, pH 4.62, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
