1RP3

Cocrystal structure of the flagellar sigma/anti-sigma complex, Sigma-28/FlgM

Experimental procedure

Experimental method	MAD
Spacegroup name	P 1 21 1
Unit cell lengths	76.388, 119.666, 100.056
Unit cell angles	90.00, 107.00, 90.00

Refinement procedure

Resolution	29.920 - 2.300
R-factor	0.242
Rwork	0.242
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.100
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	76085
<I/σ(I)>	16	2.7
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	16.2% PEG 8000, 0.09M SODIUM CACODYLATE, 0.18M CALCIUM ACETATE, 3% ISOPROPANOL, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 6.50