1RP1

DOG PANCREATIC LIPASE RELATED PROTEIN 1

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	54.026, 150.748, 132.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 2.100
R-factor	0.195
Rwork	0.195
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1thg
RMSD bond length	0.012
RMSD bond angle	26.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.067	0.368
Number of reflections	30017
<I/σ(I)>	11.2	3
Completeness [%]	97.8	94.6
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.2		drop was diluted with an equal volume of the reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.5 (mg/ml)
2	1	reservoir	PEG8000	20 (%)
3	1	reservoir	potassium phosphate	25 (mM)