1RMR
Crystal Structure of Schistatin, a Disintegrin Homodimer from saw-scaled Viper (Echis carinatus) at 2.5 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.541 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 91.645, 91.645, 55.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.19216 |
Rwork | 0.190 |
R-free | 0.22974 |
Structure solution method | MIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.333 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 3943 | |
<I/σ(I)> | 17.8 | 2.7 |
Completeness [%] | 55 | |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | ammonium sulfate, cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |