1RLA

THREE-DIMENSIONAL STRUCTURE OF RAT LIVER ARGINASE, THE BINUCLEAR MANGANESE METALLOENZYME OF THE UREA CYCLE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-09-17
Detector	RIGAKU
Spacegroup name	P 32
Unit cell lengths	88.500, 88.500, 106.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.178
Rwork	0.178
R-free	0.22900
RMSD bond length	0.011 *
RMSD bond angle	1.600 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100 *
Rmerge	0.056	0.365 *
Total number of observations	147454 *
Number of reflections	46162 *
Completeness [%]	91.6 *	58.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	4 *	Kanyo, Z.F., (1992) J.Mol.Biol., 224, 1175. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	drop	Bicine	50 (mM)
3	1	drop		1 (mM)
4	1	reservoir	PEG8000	14 (%(w/v))
5	1	reservoir	Bicine	50 (mM)
6	1	reservoir		1 (mM)
7	1	reservoir	sodium azide	0.05 (%(w/v))