1RL6
RIBOSOMAL PROTEIN L6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 71.800, 71.800, 124.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.257 * |
| Rwork | 0.257 |
| R-free | 0.30200 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | |
| Number of reflections | 13166 | |
| Completeness [%] | 97.5 | 97.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 | pH 7.6 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NA/K PHOSPHATE | ||
| 2 | 1 | 1 | BETA MERCAPTOETHANOL | ||
| 3 | 1 | 1 | 1,4-DIOXANE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | sodium potassium phosphate | 0.6 (M) | |
| 3 | 1 | drop | beta-mercaptoethanol | 3 (mM) | |
| 4 | 1 | drop | 1,4-dioxane | 3 (%(v/v)) | |
| 5 | 1 | reservoir | sodium potassium phospahte | 1.6-1.8 (M) | |
| 6 | 1 | reservoir | beta-mercaptoethanol | 3 (mM) | |
| 7 | 1 | reservoir | 1,4-dioxane | 3 (%) |
