1RKY
PPLO + Xe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.552 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.144, 66.702, 108.620 |
| Unit cell angles | 90.00, 119.24, 90.00 |
Refinement procedure
| Resolution | 31.470 - 1.680 |
| R-factor | 0.151 |
| Rwork | 0.151 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PPLO 1N9E chain A |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.403 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.460 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.090 | |
| Number of reflections | 99719 | |
| <I/σ(I)> | 11.9 | 2.1 |
| Completeness [%] | 87.7 | 84.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 35% MPD, Imidazole pH 8.0, 200mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 300K. CRYSTAL EXPOSED TO XENON AT 300 PSI FOR 90 SECONDS THEN FROZEN |
