1RKQ
Crystal structure of HAD-like phosphatase yidA from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-08-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.120, 54.871, 67.725 |
| Unit cell angles | 112.58, 96.37, 106.79 |
Refinement procedure
| Resolution | 24.500 - 1.400 |
| Rwork | 0.177 |
| R-free | 0.21300 |
| Structure solution method | Single Crystal SIRAS by RIP |
| Starting model (for MR) | Experimental Phasing by single crystal SIRAS (Radiation damage). |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.043 | 0.370 |
| Number of reflections | 106331 | |
| <I/σ(I)> | 30.9 | 2.58 |
| Completeness [%] | 95.9 | 93.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, Magnesium Chloride,, VAPOR DIFFUSION, HANGING DROP, temperature 277K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, Magnesium Chloride,, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
