1RK3
crystal structure of the rat vitamin D receptor ligand binding domain complexed with 1,25-dihydroxyvitamin D3 and a synthetic peptide containing the NR2 box of DRIP 205
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-24 |
Detector | BRUKER PROTEUM R |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.726, 44.154, 42.072 |
Unit cell angles | 90.00, 96.13, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.19303 |
Rwork | 0.190 |
R-free | 0.24255 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1rjk |
RMSD bond length | 0.016 |
RMSD bond angle | 1.384 |
Data reduction software | SAINT |
Data scaling software | LSCALE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.440 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 14475 | |
<I/σ(I)> | 10.9 | 2.7 |
Completeness [%] | 99.0 | 95 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 4000, MOPS, ammonium citrate, isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |