1RJ8

The crystal structure of TNF family member EDA-A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-01-24
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.723, 161.337, 51.023
Unit cell angles	90.00, 105.24, 90.00

Refinement procedure

Resolution	30.000 - 2.230
R-factor	0.2043
Rwork	0.200
R-free	0.24100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EDA-A1 trimer containing residues 248-307 319-333 347-390
RMSD bond length	0.007
RMSD bond angle	1.160 *
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.1.07)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.230	2.230
Rmerge	0.030	0.079 *
Total number of observations	101523 *
Number of reflections	37259
<I/σ(I)>	23	9.5
Completeness [%]	96.8	93.1 *
Redundancy	2.7	2.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	292	0.2M NaCl, 0.1M Hepes, 25% Peg 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 19K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG3350	25 (%)
3	1	reservoir		0.2 (M)
4	1	reservoir	HEPES	0.1 (M)	pH7.5