1RJ7
Crystal structure of EDA-A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.657, 279.687, 54.175 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.20127 |
| Rwork | 0.194 |
| R-free | 0.26100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | truncated model of Apo2L/TRAIL from 1d0g |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.07) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.081 | 0.239 * |
| Total number of observations | 215586 * | |
| Number of reflections | 66596 * | |
| <I/σ(I)> | 8.6 | 3 |
| Completeness [%] | 94.4 * | 93.1 * |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 19 * | 20% PEG 3350, 0.2M di-sodium hydrogen phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.5 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 20 (%) | |
| 3 | 1 | reservoir | di-sodium hydrogen phosphate | 0.2 (M) |
