1RHY

Crystal structure of Imidazole Glycerol Phosphate Dehydratase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09-15
Detector	MARRESEARCH
Wavelength(s)	1.0086, 1.0078, 0.9150
Spacegroup name	P 21 3
Unit cell lengths	105.275, 105.275, 105.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.550 - 2.300
R-factor	0.1891
Rwork	0.189
R-free	0.22800
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Number of reflections	17324
<I/σ(I)>	17.8
Completeness [%]	98.7
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	ammonium sulfate, sodium acetate,, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K