1RHO
STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-28 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 156.570, 156.570, 132.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.29800 |
| Structure solution method | MIR WITH ANOMALOUS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.254 |
| Total number of observations | 161161 * | |
| Number of reflections | 20345 | |
| <I/σ(I)> | 25.4 | 8.7 |
| Completeness [%] | 92.9 | 95.3 |
| Redundancy | 7.9 * | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | 2 M NH4 SO4 0.1 M TRIS PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 2 (M) | |
| 10 | 1 | reservoir | Tris | 0.1 (M) | |
| 11 | 1 | reservoir | DTT | 1 (mM) | |
| 12 | 1 | reservoir | sodium azide | 1 (mM) | |
| 2 | 1 | drop | Tris | 0.1 (M) | |
| 3 | 1 | drop | DTT | 1 (mM) | |
| 4 | 1 | drop | sodium azide | 1 (mM) | |
| 5 | 1 | drop | protain | 10 (mg/ml) | |
| 6 | 1 | drop | Tris | 5 (mM) | |
| 7 | 1 | drop | DTT | 1 (mM) | |
| 8 | 1 | drop | sodium azide | 1 (mM) | |
| 9 | 1 | reservoir | ammonium sulfate | 2 (M) |
