1RHL

RIBONUCLEASE T1 COMPLEXED WITH 2'GMP/G23A MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	40.440, 48.230, 50.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.950
R-factor	0.164
Rwork	0.164
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rga
RMSD bond length	0.006
RMSD bond angle	24.930 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.040
High resolution limit [Å]	1.950	1.950
Rmerge	0.126	0.475
Total number of observations	50578 *
Number of reflections	7378
<I/σ(I)>	10.7	3.3
Completeness [%]	96.2	94.1
Redundancy	6.84	6.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.2		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	2'-GMP	2 (mM)
3	1	reservoir		2 (mM)
4	1	reservoir	MPD	50 (%)	pH4.2