1RHC
F420-dependent secondary alcohol dehydrogenase in complex with an F420-acetone adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2003-01-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.04 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.824, 156.316, 60.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.800 |
| R-factor | 0.158 |
| Rwork | 0.158 |
| R-free | 0.18800 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 45082 | |
| <I/σ(I)> | 21 | 3.9 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | PEG 8000, sodium chloride, sodium citrate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
