1RH7
Crystal Structure of Resistin-like beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.06975 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.604, 86.016, 283.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.960 - 3.106 |
| R-factor | 0.20431 |
| Rwork | 0.201 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.557 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.145 | 0.325 |
| Number of reflections | 13231 | |
| <I/σ(I)> | 20 | 9.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 2.5M NaCl, 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
