1RH6
Bacteriophage Lambda Excisionase (Xis)-DNA Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.10 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.215, 72.691, 38.801 |
Unit cell angles | 90.00, 104.11, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.19293 |
Rwork | 0.191 |
R-free | 0.22553 |
Structure solution method | MIR |
RMSD bond length | 0.025 |
RMSD bond angle | 2.582 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.780 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.135 |
Number of reflections | 32190 | |
<I/σ(I)> | 19.8 | 8.2 |
Completeness [%] | 93.1 | 95.8 |
Redundancy | 93.1 | 95.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | NaOAc, imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaOAc | ||
2 | 1 | 1 | imidazole | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | NaOAc | ||
5 | 1 | 2 | imidazole | ||
6 | 1 | 2 | H2O |