1RFZ
Structure of Protein of Unknown Function from Bacillus stearothermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-06 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97956, 0.97943, 0.9647 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 80.824, 80.824, 236.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.770 - 2.800 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.107 | 0.792 |
| Number of reflections | 22606 | |
| Completeness [%] | 100 | |
| Redundancy | 15.5 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | ammonium sulfate, BIS-TRIS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
