1RFS
RIESKE SOLUBLE FRAGMENT FROM SPINACH
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.050, 31.870, 35.790 |
| Unit cell angles | 95.60, 106.10, 117.30 |
Refinement procedure
| Resolution | 20.000 - 1.830 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.920 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.048 | 0.108 |
| Total number of observations | 28882 * | |
| Number of reflections | 8758 | |
| <I/σ(I)> | 10.1 | 6.5 |
| Completeness [%] | 89.7 | 57.3 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 2 | 1 | reservoir | ammonium acetate | 100-200 (mg/ml) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | drop | sodium acetate | 50 (mM) | |
| 5 | 1 | drop | ammonium acetate | 50-100 (mg/ml) | |
| 6 | 1 | drop | PEG4000 | 15 (%) | |
| 7 | 1 | drop | protein | 15 (mg/ml) | |
| 8 | 1 | drop | MOPS | 10 (mM) |
