1RFJ
Crystal Structure of Potato Calmodulin PCM6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.440, 68.980, 109.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.950 - 2.000 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.129 |
| Number of reflections | 12583 | |
| <I/σ(I)> | 23.5 | 6.1 |
| Completeness [%] | 95.1 | 88.9 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 277 | MPD, CaCl2, NaAc-HAc, pH 3.90, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
