1RFG
Crystal Structure of Human Purine Nucleoside Phosphorylase Complexed with Guanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 104 |
| Detector technology | CCD |
| Collection date | 2003-06-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.4310 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.380, 139.380, 160.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.900 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pwy |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.003 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.224 | 3.010 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.043 | 0.214 |
| Number of reflections | 14265 | |
| Completeness [%] | 95.8 | 97.6 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | AMMONIUM SULFATE, pH 5.30, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
