1RCY
RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-10-20 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.510, 60.670, 38.140 |
| Unit cell angles | 90.00, 108.42, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.25200 |
| Structure solution method | MULTIWAVELENGTH ANOMALOUS DISPERSION |
| RMSD bond length | 0.006 |
| RMSD bond angle | 27.559 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.050 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.020 | 0.038 |
| Total number of observations | 14468 * | |
| Number of reflections | 9167 | |
| <I/σ(I)> | 23.5 | 10.1 |
| Completeness [%] | 82.0 | 63 |
| Redundancy | 1.5 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 4 * | Djebli, A., (1992) J.Mol.Biol., 227, 581. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 50 (mM) | |
| 3 | 1 | drop | lithium chloride | 100 (mM) | |
| 4 | 1 | drop | PEG8000 | 12.5 (%(w/v)) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 6 | 1 | reservoir | lithium chloride | 200 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 25 (%) |
