1RCU
X-RAY STRUCTURE OF TM1055 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET VT76
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97879, 0.97907, 0.98027 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.748, 90.438, 83.210 |
| Unit cell angles | 90.00, 94.56, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.096 |
| Number of reflections | 43759 |
| Completeness [%] | 97.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 0.2M sodium Formate, 0.05M Tris, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP |
