1RCO
SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR D-XYLULOSE-2,2-DIOL-1,5-BISPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 279 |
| Detector technology | IMAGE PLATE |
| Collection date | 1990-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 220.600, 221.700, 115.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.300 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ruc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.147 | 0.413 |
| Total number of observations | 1519008 * | |
| Number of reflections | 217862 | |
| <I/σ(I)> | 3.4 | 1.3 |
| Completeness [%] | 62.5 | 62.5 |
| Redundancy | 7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 4 * | 15% PEG 4000, 25 MM HEPES PH 7.8, 10 MM CACL2, 0.2 M NACL, 50 MM NAHCO3, 20 MM XUBP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 60 (mg/ml) | |
| 2 | 1 | drop | HEPES | 25 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | XuBP | 20 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 15-16 (%) | |
| 7 | 1 | reservoir | HEPES | 25 (mM) | |
| 8 | 1 | reservoir | 0.2 (M) | ||
| 9 | 1 | reservoir | 50 (mM) |
