1RC0
Human GAR Tfase complex structure with polyglutamated 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-19 |
Detector | ADSC QUANTUM 9 |
Wavelength(s) | 0.954 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 126.305, 126.305, 93.598 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.580 - 2.050 |
R-factor | 0.22319 |
Rwork | 0.222 |
R-free | 0.24257 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1njs |
RMSD bond length | 0.010 |
RMSD bond angle | 1.204 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.580 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 54005 | |
<I/σ(I)> | 26.7 | 2.8 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 3.55 | 3.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277 | isoproponal, Calcium Cloride, Sodum Acetate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |