1RBO

SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	279
Detector technology	IMAGE PLATE
Collection date	1989-11
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	157.200, 157.200, 201.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.154
Rwork	0.154
R-free	0.17100
Structure solution method	ISOMORPHOUS WITH 8RUC
RMSD bond length	0.010
RMSD bond angle	24.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.059
Total number of observations	488333 *
Number of reflections	108532
<I/σ(I)>	9.8
Completeness [%]	90.7	100
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4		1.7 M (NH4)2SO4, 50 MM PHOSPHATE, PH 7.4 3 MM MGCL2, 25 MM NAHCO3, 10 MM 4CABP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	spinach Rubisco	100 (mg/ml)
2	1	drop		3 (mM)
3	1	drop		25 (mM)
4	1	drop	4-CABP	10 (mM)
5	1	drop	phosphate	50 (mM)
6	1	reservoir	ammonium sulfate	1.7 (M)
7	1	reservoir	phosphate	50 (mM)