1R95
Crystal Structure of IscA (native)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 |
| Unit cell lengths | 55.527, 55.527, 159.037 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 * - 2.600* |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.25600 |
| Structure solution method | SIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | 2.750 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.066 * | 0.479 * |
| Total number of observations | 77724 * | |
| Number of reflections | 8034 | |
| <I/σ(I)> | 58 | 5.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.7 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 298 | dibasic ammonium phosphate, imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | ferrous ammonium sulfate | 1.1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | dibasic ammonium phosphate | 0.8-1.0 (M) | |
| 5 | 1 | reservoir | imidazole | 0.1 (M) | pH8.0 |
