1R8X
Crystal Structure of Mouse Glycine N-Methyltransferase (Tetragonal Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.700, 70.700, 266.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.500 - 2.950 |
| R-factor | 0.21048 |
| Rwork | 0.207 |
| R-free | 0.27266 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xva |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.167 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 14973 | |
| <I/σ(I)> | 6.7 | 1.8 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | PEG4000, isopropanol, Tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
