1R8G
Structure and function of YbdK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97925 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.470, 100.320, 71.370 |
Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
Rwork | 0.189 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.470 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.860 | 2.200 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.061 | 0.103 |
Number of reflections | 62133 | |
<I/σ(I)> | 15.34 | 6.71 |
Completeness [%] | 87.8 | 49.4 |
Redundancy | 3.8 | 0.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2M NaCl, 20% w/v PEG 3350, 20mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |