1R7J
Crystal structure of the DNA-binding protein Sso10a from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.940, 72.300, 30.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.860 - 1.470 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.24400 |
Structure solution method | Sulfur-SAS on Cr X-ray source |
RMSD bond length | 0.004 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.860 | 1.520 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.042 | 0.264 |
Number of reflections | 21672 | |
<I/σ(I)> | 18.4 | 4.6 |
Completeness [%] | 96.5 | 69.2 |
Redundancy | 12.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 3350, potassium fluoride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |