1R7A

Sucrose Phosphorylase from Bifidobacterium adolescentis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2003-05-11
Detector	MARRESEARCH
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	55.040, 123.360, 151.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.770
R-factor	0.164
Rwork	0.164
R-free	0.19100 *
Structure solution method	SIRAS
RMSD bond length	0.006 *
RMSD bond angle	1.400 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.300	1.660
High resolution limit [Å]	1.580	1.580
Rmerge	0.109 *	0.530 *
Number of reflections	138899
<I/σ(I)>	4.3	0.9
Completeness [%]	97.8	85.2 *
Redundancy	3.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1 *	25 *	PEG 4000, sodium acetate, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.1 *	25 *	PEG 4000, sodium acetate, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.1 *	25 *	PEG 4000, sodium acetate, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5-1.0 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.1
3	1	reservoir	PEG4000	27 (%(w/v))
4	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
5	1	reservoir	sodium acetate	0.1 (M)