1R6X

The Crystal Structure of a Truncated Form of Yeast ATP Sulfurylase, Lacking the C-Terminal APS Kinase-like Domain, in complex with Sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.90
Spacegroup name	P 21 21 21
Unit cell lengths	50.186, 59.111, 131.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.860 - 1.400
R-factor	0.197
Rwork	0.197
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j70
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.490
High resolution limit [Å]	1.400	1.400
Number of reflections	67770
Completeness [%]		40.5
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.9M (NH4)SO4, 0.1M MES, 0.01 M Cobaltous chloride, 8% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K