1R6N

HPV11 E2 TAD complex crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-01
Detector	BRANDEIS - B4
Wavelength(s)	1.000
Spacegroup name	P 41
Unit cell lengths	60.456, 60.456, 82.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.2095
Rwork	0.209
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HPV11 E2 TAb apo (1R6K)
RMSD bond length	0.007
RMSD bond angle	1.190
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.079	0.474
Total number of observations	70999 *
Number of reflections	11028 *
<I/σ(I)>	27.47	2.01
Completeness [%]	97.9	86.4
Redundancy	6.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *	295	Na Citrate, MPD, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		60 (mM)
2	1	drop	His-Lys-TAD	10 (mg/ml)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.5
4	1	reservoir	MPD	35 (%)