1R6L

Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2003-05-28
Detector	MACSCIENCE
Wavelength(s)	0.9793, 0.9792, 0.9716
Spacegroup name	H 3 2
Unit cell lengths	111.139, 111.139, 115.841
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 1.900
R-factor	0.221
Rwork	0.221
R-free	0.24500 *
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	23.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.047 *	0.224 *
Total number of observations	415708 *
Number of reflections	21859
Completeness [%]	99.7	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	18 *	Ammonium sulfate, CHES, sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)	pH8.0
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	ammonium sulfate	1.2 (M)
6	1	reservoir		0.1 (M)
7	1	reservoir	CHES	0.2 (M)	pH9.5