1R6J

Ultrahigh resolution Crystal Structure of syntenin PDZ2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.85
Spacegroup name	P 1 21 1
Unit cell lengths	25.876, 39.544, 32.276
Unit cell angles	90.00, 109.64, 90.00

Refinement procedure

Resolution	10.000 - 0.730
Rwork	0.075
R-free	0.08660
Structure solution method	AB INITIO
RMSD bond length	0.019
RMSD bond angle	0.033
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	0.760
High resolution limit [Å]	0.730	0.730
Rmerge	0.060	0.387
Number of reflections	71347
Completeness [%]	83.9	9.6
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	PEG 4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K