1R6B
High resolution crystal structure of ClpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 |
| Unit cell lengths | 124.110, 124.110, 97.049 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.23504 |
| Rwork | 0.234 |
| R-free | 0.27784 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.144 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.032 | 0.349 |
| Number of reflections | 37627 | |
| <I/σ(I)> | 21.9 | 1.36 |
| Completeness [%] | 0.9 | 0.79 |
| Redundancy | 2.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | hepes, potassium chloride, magnesium chloride, isopropanol, PEG 4000, glycerol, ADP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
