1R5T

The Crystal Structure of Cytidine Deaminase CDD1, an Orphan C to U editase from Yeast

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-17
Detector	APS-1
Wavelength(s)	1.2574, 1.2831, 1.2828
Spacegroup name	C 2 2 21
Unit cell lengths	78.520, 86.370, 156.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.050 - 2.000
R-factor	0.188
Rwork	0.188
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	63560
<I/σ(I)>	17.7	2.1
Completeness [%]	91.5	74.8
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG 5000 MME, ammonium chloride, sodium succinate, DTT, sodium azide, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG 5000 MME, ammonium chloride, sodium succinate, DTT, sodium azide, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG 5000 MME, ammonium chloride, sodium succinate, DTT, sodium azide, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K