1R5Q
Crystal Structure Analysis of Kai A from PCC7120
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2002-11-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 57.299, 57.299, 137.515 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.420 - 2.000 |
| Rwork | 0.241 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 9566 | |
| Completeness [%] | 98.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MgSO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MgSO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
