1R5O

crystal structure analysis of sup35 complexed with GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-10
Detector	MARRESEARCH
Wavelength(s)	0.9724
Spacegroup name	P 43 21 2
Unit cell lengths	82.524, 82.524, 168.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.26836
Rwork	0.266
R-free	0.31959
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r5b
RMSD bond length	0.012
RMSD bond angle	1.377
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.140	0.140
Number of reflections	11894
<I/σ(I)>	5.2	0.7
Completeness [%]	98.3	98.3
Redundancy		7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	HEPES, Ethylene Glycol, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K