1R56

UNCOMPLEXED URATE OXIDASE FROM ASPERGILLUS FLAVUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	283
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	82.590, 141.843, 134.959
Unit cell angles	90.00, 92.69, 90.00

Refinement procedure

Resolution	10.000 - 2.300
Rwork	0.162
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UOX
RMSD bond length	0.012
RMSD bond angle	1.275
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000
High resolution limit [Å]	2.300	2.300
Rmerge		0.426
Number of reflections	136450
<I/σ(I)>	12.3	3.6
Completeness [%]	99.2	98.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	291	8.5MG/ML PROTEIN, 5-7% W/V PEG 8000, 100MM TRIS/HCL, temperature 291K, pH 8.00