1R55
Crystal structure of the catalytic domain of human ADAM 33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.990, 65.205, 99.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.580 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 24596 | |
| Completeness [%] | 93.5 | 56.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 277 | PEG 800, sodium chloride, caps, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
