1R4A
Crystal Structure of GTP-bound ADP-ribosylation Factor Like Protein 1 (Arl1) and GRIP Domain of Golgin245 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9720 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.083, 86.924, 86.246 |
| Unit cell angles | 118.11, 99.65, 95.47 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2508 |
| Rwork | 0.246 |
| R-free | 0.26000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ksg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 * | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 | 0.539 |
| Number of reflections | 55268 | |
| <I/σ(I)> | 8.4 | 1.88 |
| Completeness [%] | 97.1 * | 56.5 |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | PEG3350, potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 17-18 (%(w/v)) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 20 (mM) | pH8.0 |
