1R4A

Crystal Structure of GTP-bound ADP-ribosylation Factor Like Protein 1 (Arl1) and GRIP Domain of Golgin245 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-10
Detector	MARRESEARCH
Wavelength(s)	0.9720
Spacegroup name	P 1
Unit cell lengths	42.083, 86.924, 86.246
Unit cell angles	118.11, 99.65, 95.47

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.2508
Rwork	0.246
R-free	0.26000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ksg
RMSD bond length	0.011
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000 *	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.075	0.539
Number of reflections	55268
<I/σ(I)>	8.4	1.88
Completeness [%]	97.1 *	56.5
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	PEG3350, potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	reservoir	PEG3350	17-18 (%(w/v))
3	1	reservoir		200 (mM)
4	1	reservoir	Tris-HCl	20 (mM)	pH8.0