1R1Z

The Crystal structure of the Carbohydrate recognition domain of the glycoprotein sorting receptor p58/ERGIC-53 reveals a novel metal binding site and conformational changes associated with calcium ion binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 1
Unit cell lengths	44.351, 81.068, 82.306
Unit cell angles	91.05, 94.14, 94.99

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.22297
Rwork	0.223
R-free	0.24100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gv9
RMSD bond length	0.013 *
RMSD bond angle	1.240 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.092 *	0.334 *
Number of reflections	42111 *
<I/σ(I)>	13	1.7
Completeness [%]	94.6 *	95.3 *
Redundancy	2.3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG8000, Calcium Chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	10 (mM)	pH7.5
3	1	reservoir	PEG8000	25 (%(w/v))
4	1	reservoir		2 (mM)
5	1	reservoir	sodium HEPES	100 (mM)	pH7.5