1R1Z
The Crystal structure of the Carbohydrate recognition domain of the glycoprotein sorting receptor p58/ERGIC-53 reveals a novel metal binding site and conformational changes associated with calcium ion binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.351, 81.068, 82.306 |
| Unit cell angles | 91.05, 94.14, 94.99 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.22297 |
| Rwork | 0.223 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gv9 |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.240 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.092 * | 0.334 * |
| Number of reflections | 42111 * | |
| <I/σ(I)> | 13 | 1.7 |
| Completeness [%] | 94.6 * | 95.3 * |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG8000, Calcium Chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG8000 | 25 (%(w/v)) | |
| 4 | 1 | reservoir | 2 (mM) | ||
| 5 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
