1QZX

Crystal structure of the complete core of archaeal SRP and implications for inter-domain communication

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9740
Spacegroup name	I 4
Unit cell lengths	197.906, 197.906, 64.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 4.000
Rwork	0.313
R-free	0.38300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j8m
RMSD bond length	0.016
RMSD bond angle	1.930 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	BEAST
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	4.220
High resolution limit [Å]	4.000	4.000
Rmerge	0.083 *	0.484 *
Number of reflections	9576 *
<I/σ(I)>	4.8	1.5
Completeness [%]	89.0 *	97.9 *
Redundancy	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Rosendal, K.R., (2004) Acta Crystallogr.,Sect.D, 60, 140. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	PEG8000	2-4 (%)
3	1	reservoir		1-1.2 (M)