1QZW
Crystal structure of the complete core of archaeal SRP and implications for inter-domain communication
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9763 |
Spacegroup name | P 31 |
Unit cell lengths | 137.763, 137.763, 307.894 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 4.100 |
Rwork | 0.340 |
R-free | 0.38700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1J8M |
RMSD bond length | 0.012 |
RMSD bond angle | 2.260 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.320 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.087 * | 0.481 * |
Number of reflections | 49660 * | |
<I/σ(I)> | 4 | 1.5 |
Completeness [%] | 97.2 * | 98.1 * |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | Rosendal, K.R., (2004) Acta Crystallogr.,Sect.D, 60, 140. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ammonium sulphate | ||
2 | 1 | 1 | sodium chloride | ||
3 | 1 | 1 | sodium acetate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Ammonium sulphate | ||
6 | 1 | 2 | sodium acetate | ||
7 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.26 (M) | pH4.5 |
3 | 1 | reservoir | 200 (mM) |