1QZU
crystal structure of human phosphopantothenoylcysteine decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9876 |
| Spacegroup name | H 3 |
| Unit cell lengths | 124.780, 124.780, 153.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.480 - 2.910 |
| R-factor | 0.293 |
| Rwork | 0.293 |
| R-free | 0.34200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e20 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.045 | 0.228 |
| Number of reflections | 18689 | |
| <I/σ(I)> | 22.6 | 3.4 |
| Completeness [%] | 96.2 | 98.3 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | ammonium sulphate, dioxane, 2-(N-Morpholino)ethane sulfonic acid, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
